Anti-Tom20/Tomm20; clone 2F8.1

Code: MABT166 D2-231

Analysis Note

ControlHeLa cell lysate

Application

Anti-Tom20/Tomm20, clone 2F8.1 detects levels of Tom20/Tomm20 proteins & has been published & v...


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Analysis Note

ControlHeLa cell lysate

Application

Anti-Tom20/Tomm20, clone 2F8.1 detects levels of Tom20/Tomm20 proteins & has been published & validated for use in WB & ICC.

Research CategoryCell Structure

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Tom20/Tomm20 in A431, NIH/3T3, and HeLa cells.

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mitochondrial import receptor subunit TOM20 homolog (TOM20 or TOMM20), also known as mitochondrial 20 kDa outer membrane protein or outer mitochondrial membrane receptor Tom20, is a central component of the pre-protein translocase complex of the outer mitochondrial membrane (TOM complex) which is responsible for the recognition and translocation of cytosolically synthesized mitochondrial pre-proteins. The TOM complex consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). TOM20 interacts with TOM22 and facilitates the movement of pre-proteins into the TOM40 translocation pore. Also interacts with APEX1.

Immunogen

GST-tagged recombinant protein corresponding to human Tom20/Tomm20.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Tom20/Tomm20 in 200 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 16 kDa observed

antibody formpurified antibody
antibody product typeprimary antibodies
biological sourcemouse
clone2F8.1, monoclonal
Gene Informationhuman ... TOMM20(9804)
isotypeIgG1κ
NCBI accession no.NP_055580
Quality Level100
shipped inwet ice
species reactivitymouse, human
technique(s)immunocytochemistry: suitable, western blot: suitable
UniProt accession no.Q15388
This product has met the following criteria to qualify for the following awards:



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